DDRGK1 Rekombinanter Antikörper

DDRGK1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251582G2

Kat-Nr. : 86667-2-PBS

Synonyme

C20orf116, Dashurin, DDRGK domain containing 1, DDRGK domain-containing protein 1, UFBP1

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  WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86667-2-RR (DDRGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

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  WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86667-2-RR (DDRGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86667-2-RR (same clone as 86667-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (86667-2-RR, Clone: 251582G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02579-1

  The mean DDRGK1 concentration was determined to be 109.2 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 36.0 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02579-1

  Cytometric bead array standard curve of MP02579-1, DDRGK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86667-2-PBS. Detection antibody: 86667-1-PBS. Standard: Ag13742. Range: 0.469-60 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86667-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA DDRGK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDRGK1 fusion protein Ag13742
• Vollständiger Name: DDRGK domain containing 1
• Berechnetes Molekulargewicht: 314 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-46 kDa
• GenBank-Zugangsnummer: BC000643
• Gene symbol: DDRGK1
• Gene ID (NCBI): 65992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531)