DDX1 Monoklonaler Antikörper

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• KD/KO Validated

DDX1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10G4

Kat-Nr. : 67991-1-PBS

Synonyme

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

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  WB analysis using 67991-1-Ig (same clone as 67991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67991-1-Ig (same clone as 67991-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67991-1-Ig (same clone as 67991-1-PBS)

  WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67991-1-PBS bindet in WB, IHC, Indirect ELISA DDX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX1 fusion protein Ag16774
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• Berechnetes Molekulargewicht: 740 aa, 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC012132
• Gene symbol: DDX1
• Gene ID (NCBI): 1653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.