DDX10 Polyklonaler Antikörper

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• KD/KO Validated

DDX10 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 17857-1-AP

Synonyme

DDX10, DEAD box protein 10, HRH J8

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• 

  WB analysis of SMMC-7721 using 17857-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SMMC-7721-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17857-1-AP bindet in WB, IHC, IF, CoIP, ELISA DDX10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX10 fusion protein Ag12317
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Berechnetes Molekulargewicht: 875 aa, 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC091521
• Gene symbol: DDX10
• Gene ID (NCBI): 1662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Cell Int; DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35.; Authors - Xin Zhou ; KD Validated
human	WB	Biochem Biophys Res Commun; Epigenetic down-regulated DDX10 promotes cell proliferation through Akt/NF-κB pathway in ovarian cancer.; Authors - Muhuizi Gai ; KD Validated
human	WB	Front Immunol; Circular RNA TAF4B targeting MFN2 accelerates cell growth in bladder cancer through p27 depression and AKT activation; Authors - Xiaoting Zhang
human,mouse	WB,IHC,IF,CoIP	Research (Wash D C); DDX10 Exacerbates Exosomal PD-L1-Dependent T Cell Exhaustion via Phase Separation of Rab27b in Oral Squamous Cell Carcinoma; Authors - Bowen Li ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wael (Verified Customer) (05-24-2023)

We detected the DDX10 relative abundance in both the nuclear and cytoplasmic fractions

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX10 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17857-1-AP)