DDX10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDX10 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17857-1-PBS

Synonyme

---

• 

  WB analysis of SMMC-7721 using 17857-1-AP (same clone as 17857-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17857-1-AP (same clone as 17857-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

17857-1-PBS bindet in WB, IHC, Indirect ELISA DDX10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX10 fusion protein Ag12317
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Berechnetes Molekulargewicht: 875 aa, 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC091521
• Gene symbol: DDX10
• Gene ID (NCBI): 1662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).