DDX20 Polyklonaler Antikörper

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• KD/KO Validated

DDX20 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11324-1-AP

Synonyme

Component of gems 3, DDX20, DEAD box protein 20, DEAD box protein DP 103, DP103, Gemin 3, GEMIN3

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Galerie der Validierungsdaten

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  WB analysis using 11324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11324-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11324-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11324-1-AP

  IP result of anti-DDX20(IP:11324-1-AP, 4ug; Detection:11324-1-AP 1:2000) with HeLa cells lysate 1880 ug.

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  IHC staining of human breast cancer using 11324-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11324-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11324-1-AP (DDX20 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11324-1-AP

  Immunofluorescent analysis of HepG2 cells, using DDX20 antibody 11324-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 11324-1-AP

  Immunofluorescent analysis of Hela cells, using DDX20 antibody 11324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 11324-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX20 (11324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11324-1-AP bindet in WB, IP, IHC, IF, FC, ELISA DDX20 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX20 fusion protein Ag1863
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
• Berechnetes Molekulargewicht: 824 aa, 92 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC011556
• Gene symbol: DDX20
• Gene ID (NCBI): 11218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), also known as DP103 or Gemin3, is a member of the DEAD box protein family expressed ubiquitously. DEAD family proteins use energy from ATP hydrolysis for RNA chaperoning and RNPase activity (PMID: 27121695). As a core member of the survival motor neuron (SMN) complex, DDX20 participate in small nuclear ribonucleoprotein (snRNP) biogenesis. Second, DDX20 have direct roles in gene expression in view of its implication in transcription and post-transcriptional gene silencing. Addition, the false expression of DDX20 could have deleterious effects on cellular homeostasis thus leading to cancer development and progression (PMID:29523774). Anymore, DDX20 could be identified as a biomarker and metastasis-driving oncogene of human breast cancer (PMID: 25083991). The detected weight of DDX20 is slightly higher than the theoretical molecular weight that is because of phosphorylation after translation.

Publikationen

Species	Application	Title
human	WB, IHC	J Clin Invest; DEAD-box helicase DP103 defines metastatic potential of human breast cancers.; Authors - Eun Myoung Shin ; KD Validated
human	IHC	Aging (Albany NY); An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes; Authors - Fan Peng
human	WB	Comput Struct Biotechnol J; Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer.; Authors - Qingshui Wang
human	IHC	Arthritis Res Ther; Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.; Authors - Ping Li
	WB	Biosci Rep; FOXL2 Down-regulates Vitellogenin Expression at Mature Stage in Eriocheir sinensis.; Authors - Qing Li
human	IF	FEBS Lett; Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.; Authors - Dongxu Jiang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C