DDX21 Rekombinanter Antikörper

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• KD/KO Validated

DDX21 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5E7

Kat-Nr. : 80932-1-RR

Synonyme

5E7, DEAD box protein 21, DExD-box helicase 21, EC:3.6.4.13, Gu alpha

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  WB analysis using 80932-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80932-1-RR (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 80932-1-RR

  WB result of DDX21 antibody (80932-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells.

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  IF Staining of HepG2 using 80932-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 80932-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 80932-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80932-1-RR against Human DDX21 were performed. The affinity constant is 77.7 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80932-1-RR bindet in WB, IF/ICC, ELISA DDX21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDX21 fusion protein Ag0804
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC008071
• Gene symbol: DDX21
• Gene ID (NCBI): 9188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa.