DDX21 Monoklonaler Antikörper

DDX21 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9C8

Kat-Nr. : CL488-66925

Synonyme

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

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  IF Staining of HepG2 using CL488-66925

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DDX21 antibody (CL488-66925, Clone: 1B9C8 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HepG2 using CL488-66925

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human DDX21 (CL488-66925, Clone:1B9C8) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66925 bindet in IF/ICC, FC (Intra) DDX21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX21 fusion protein Ag17850
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Berechnetes Molekulargewicht: 783 aa, 87 kDa
• GenBank-Zugangsnummer: BC008071
• Gene symbol: DDX21
• Gene ID (NCBI): 9188
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.