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DDX23 Rekombinanter Antikörper

DDX23 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243147B4

Kat-Nr. : 85763-4-PBS

Synonyme

100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28

Filter:
  • Western Blot (WB) analysis of various lysates using DDX23 Recombinant antibody (85763-4-RR)

    WB analysis using 85763-4-RR (same clone as 85763-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85763-4-RR (DDX23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85763-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX23 Recombinant antibody (85763-4-RR)

    IF Staining of HeLa using 85763-4-RR (same clone as 85763-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX23 antibody (85763-4-RR, Clone: 243147B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85763-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85763-4-RR

    Affinity and Kinetic Characterization of 85763-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85763-4-RR against Human DDX23 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85763-4-PBS bindet in WB, IF/ICC, Indirect ELISA DDX23 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DDX23 fusion protein Ag0258
Vollständiger Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Berechnetes Molekulargewicht 96 kDa
Beobachtetes Molekulargewicht96-100 kDa
GenBank-ZugangsnummerBC002366
Gene symbol DDX23
Gene ID (NCBI) 9416
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23, synonyms: prp28, MGC8416, U5-100K) is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX23 is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.