DDX24 Polyklonaler Antikörper

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• KD/KO Validated

DDX24 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15769-1-PBS

Synonyme

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24

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  WB analysis using 15769-1-AP (same clone as 15769-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 15769-1-AP (DDX24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  WB analysis using 15769-1-AP (same clone as 15769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15769-1-AP (DDX24 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 15769-1-AP (same clone as 15769-1-PBS)

  WB result of DDX24 antibody (15769-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX24 transfected A549 cells. This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15769-1-AP (same clone as 15769-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15769-1-AP (DDX24 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15769-1-AP (same clone as 15769-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (15769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15769-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DDX24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX24 fusion protein Ag8407
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC008847
• Gene symbol: DDX24
• Gene ID (NCBI): 57062
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.