DDX3 Monoklonaler Antikörper

DDX3 Monoklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D3A1

Kat-Nr. : 67915-1-PBS

Synonyme

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

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  WB analysis using 67915-1-Ig (same clone as 67915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67915-1-Ig (DDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

  WB result of DDX3 antibody (67915-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells. This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67915-1-Ig (same clone as 67915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67915-1-Ig (same clone as 67915-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human DDX3 (67915-1-Ig, Clone:1D3A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67915-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67915-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA DDX3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX3 fusion protein Ag29972
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 71-73 kDa
• GenBank-Zugangsnummer: BC011819
• Gene symbol: DDX3
• Gene ID (NCBI): 1654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.