DDX39A Polyklonaler Antikörper

DDX39A Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 11723-1-AP

Synonyme

BAT1, BAT1L, DDX39, DDX39A, DDXL, DEAD box protein 39, Nuclear RNA helicase URH49, URH49

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Galerie der Validierungsdaten

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  WB analysis using 11723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11723-1-AP (DDX39A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11723-1-AP (DDX39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11723-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11723-1-AP (DDX39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11723-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11723-1-AP (DDX39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11723-1-AP

  IP Result of anti-DDX39 (IP:11723-1-AP, 4ug; Detection:11723-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IP experiment of HEK-293 using 11723-1-AP

  IP Result of anti-DDX39 (IP:11723-1-AP, 4ug; Detection:11723-1-AP 1:1000) with HEK-293 cells lysate 3680ug.

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  IHC staining of mouse kidney using 11723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11723-1-AP (DDX39A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11723-1-AP (DDX39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11723-1-AP (DDX39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11723-1-AP (DDX39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11723-1-AP (DDX39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39A antibody (11723-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 11723-1-AP

  Immunofluorescent analysis of HepG2 cells, using DDX39 antibody 11723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushodengewebe, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11723-1-AP bindet in WB, IP, IHC, IF, ELISA DDX39A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX39A fusion protein Ag2311
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
• Berechnetes Molekulargewicht: 427 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC032128
• Gene symbol: DDX39
• Gene ID (NCBI): 10212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs.Some of the motifs were known prior to the definition of the family and are responsible for binding to mRNAor ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes. This antibody may cross-react with DDX39B.

Publikationen

Species	Application	Title
mouse	WB	Nucleic Acids Res; A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing.; Authors - Teruhiko Suzuki
human	WB,IHC	Int J Biol Sci; DDX39 as a predictor of clinical prognosis and immune checkpoint therapy efficacy in patients with clear cell renal cell carcinoma.; Authors - Yewei Bao
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
human,chicken	IF,WB	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated
human	IP,WB,IF	Mol Cell; 1The RNA helicase DDX39A binds a conserved structure in chikungunya virus RNA to control infection; Authors - Iulia Tapescu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Robin (Verified Customer) (12-12-2019)

Great signal but specificity is questionable in comparison to UAP56 (ddx39B)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse fibroblasts