DDX39A Rekombinanter Antikörper

DDX39A Rekombinant Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

230375F12

Kat-Nr. : 83083-4-RR

Synonyme

BAT1, BAT1L, DDX39, DDXL, DEAD box protein 39, Nuclear RNA helicase URH49, URH49

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  WB analysis using 83083-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83083-4-RR (DDX39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83083-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39 antibody (83083-4-RR, Clone: 230375F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83083-4-RR bindet in WB, IF, ELISA DDX39A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDX39A fusion protein Ag2311
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
• Berechnetes Molekulargewicht: 427 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC032128
• Gene symbol: DDX39
• Gene ID (NCBI): 10212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.