DDX39A Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

DDX39A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230375F9

Kat-Nr. : 83083-5-RR

Synonyme

DDX39, 230375F9, ATP-dependent RNA helicase DDX39A, BAT1, BAT1L

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  WB analysis using 83083-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83083-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83083-5-RR

  WB result of DDX39A antibody (83083-5-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX39A transfected HEK-293 cells.

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  IHC staining of mouse kidney using 83083-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83083-5-RR (DDX39A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83083-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39 antibody (83083-5-RR, Clone: 230375F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 83083-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human DDX39A (83083-5-RR, Clone:230375F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83083-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83083-5-RR against Human DDX39A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83083-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA DDX39A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDX39A fusion protein Ag2311
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
• Berechnetes Molekulargewicht: 427 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC032128
• Gene symbol: DDX39
• Gene ID (NCBI): 10212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.