DDX3Y Polyklonaler Antikörper

DDX3Y Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14041-1-AP

Synonyme

ATP-dependent RNA helicase DDX3Y, DBY, DEAD box protein 3, Y-chromosomal, EC:3.6.4.13

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  WB analysis using 14041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14041-1-AP (DDX3Y antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 14041-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14041-1-AP (DDX3Y antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 14041-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14041-1-AP (DDX3Y antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14041-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14041-1-AP (DDX3Y antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14041-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14041-1-AP (DDX3Y antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DDX3Y antibody (14041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IP: See 1 publications below

Produktinformation

14041-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DDX3Y und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX3Y fusion protein Ag5110
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC034942
• Gene symbol: DDX3Y
• Gene ID (NCBI): 8653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX3Y is one member of DEAD box RNA helicases, encode by the gene locate on the chromosome Y in the azoospermia factor A region. Polyclonal anti-DDX3Y antibodies (14041-1-AP) are produced by immunizing animals with C-terminus of DDX3Y and detect an 73-kDa band. As DDX3Y has a structural homolog with DDX3X, the antibody can recognize both.

Publikationen

Species	Application	Title
human	IP	Acta Histochem; Antioncogenic roles of USP9Y and DDX3Y in lung cancer: USP9Y stabilizes DDX3Y by preventing its degradation through deubiquitination; Authors - Lei Xiu