DDX4,VASA Monoklonaler Antikörper

DDX4,VASA Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1F12C3

Kat-Nr. : 67147-2-Ig

Synonyme

DDX4, 1F12C3, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX4, VASA

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  WB analysis of SKOV-3 using 67147-2-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 67147-2-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67147-2-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67147-2-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, JAR-Zellen, NCCIT-Zellen, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

67147-2-Ig bindet in WB, IF, ELISA DDX4,VASA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX4,VASA fusion protein Ag11630
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Berechnetes Molekulargewicht: 690aa,76 kDa; 724aa,79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC047455
• Gene symbol: DDX4
• Gene ID (NCBI): 54514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publikationen

Species	Application	Title
mouse	IF	Reproduction; NR2C2 of macrophages promotes inflammation via NF-κB in LPS-induced orchitis in mice; Authors - Ziqian Min
rat	IF	Reprod Sci; The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve; Authors - Qianwen Ma
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
rat	IF	Reprod Sci; Mechanism Research of DHEA Treatment Improving Diminished Ovarian Reserve by Attenuating the AMPK-SIRT1 Signaling and Mitophagy; Authors - Qianwen Ma