DDX4,VASA Monoklonaler Antikörper

DDX4,VASA Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12C3

Kat-Nr. : 67147-2-PBS

Synonyme

DDX4, 1F12C3, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX4, VASA

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• 

  WB analysis of SKOV-3 using 67147-2-Ig (same clone as 67147-2-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 67147-2-Ig (same clone as 67147-2-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67147-2-Ig (same clone as 67147-2-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 67147-2-Ig (same clone as 67147-2-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67147-2-PBS bindet in WB, ELISA DDX4,VASA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX4,VASA fusion protein Ag11630
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Berechnetes Molekulargewicht: 690aa,76 kDa; 724aa,79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC047455
• Gene symbol: DDX4
• Gene ID (NCBI): 54514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].