DDX4,VASA Monoklonaler Antikörper

DDX4,VASA Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

1F12C3

Kat-Nr. : CL594-67147

Synonyme

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

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  IF Staining of mouse testis using CL594-67147

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 DDX4,VASA antibody (CL594-67147, Clone: 1F12C3 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-67147 bindet in IF-P DDX4,VASA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX4,VASA fusion protein Ag11630
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Berechnetes Molekulargewicht: 690aa,76 kDa; 724aa,79 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC047455
• Gene symbol: DDX4
• Gene ID (NCBI): 54514
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publikationen

Species	Application	Title
mouse	IF	Biol Reprod; ARHGEF15 in Sertoli cells contributes to germ cell development and testicular immune privilege; Authors - Feng Chen