DDX46 Rekombinanter Antikörper

DDX46 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242715E10

Kat-Nr. : 85065-3-PBS

Synonyme

242715E10, DEAD box protein 46, EC:3.6.4.13, KIAA0801, Probable ATP-dependent RNA helicase DDX46

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  WB analysis using 85065-3-RR (same clone as 85065-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85065-3-RR (DDX46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01807-1

  Sandwich ELISA standard curve of MP01807-1, Human DDX46 Recombinant Matched Antibody Pair - PBS only. 85065-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10342. 85065-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP01807-1

  The mean DDX46 concentration was determined to be 109.98 ng/mL in HEK-293 cell extract based on a 2.70 mg/mL extract load and 35.02 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

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  IHC staining of Human Hepatocellular cancer using 85065-3-RR (same clone as 85065-3-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 85065-3-RR (DDX46 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 85065-3-RR (same clone as 85065-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using DDX46 antibody (85065-3-RR, Clone: 242715E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01807-1

  Cytometric bead array standard curve of MP01807-1, DDX46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85065-3-PBS. Detection antibody: 85065-1-PBS. Standard: Ag10342. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85065-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85065-3-RR against Human DDX46 were performed. The affinity constant is 51.9 pM.

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Geprüfte Anwendungen

Produktinformation

85065-3-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test DDX46 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDX46 fusion protein Ag10342
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
• Berechnetes Molekulargewicht: 1031 aa, 117 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC012304
• Gene symbol: DDX46
• Gene ID (NCBI): 9879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.