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DDX47 Polyklonaler Antikörper

DDX47 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17795-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using DDX47 Polyclonal antibody (17795-1-AP)

    WB analysis using 17795-1-AP (same clone as 17795-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17795-1-AP (DDX47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17795-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DDX47 Polyclonal antibody (17795-1-AP)

    IF Staining of U2OS using 17795-1-AP (same clone as 17795-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (17795-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17795-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17795-1-PBS bindet in WB, IF/ICC, Indirect ELISA DDX47 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DDX47 fusion protein Ag11953
Vollständiger Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Berechnetes Molekulargewicht 455 aa, 51 kDa
Beobachtetes Molekulargewicht48 kDa
GenBank-ZugangsnummerBC068009
Gene symbol DDX47
Gene ID (NCBI) 51202
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).