DDX47 Rekombinanter Antikörper

DDX47 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240193C4

Kat-Nr. : 83287-3-RR

Synonyme

MSTP162, HQ0256, EC:3.6.4.13, E4 DBP, DEAD box protein 47

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  WB analysis using 83287-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83287-3-RR (DDX47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83287-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 83287-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U2OS using 83287-3-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX47 Recombinant antibody (83287-3-RR, Clone:240193C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83287-3-RR bindet in WB, IF/ICC, FC (Intra), ELISA DDX47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDX47 fusion protein Ag11953
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• Berechnetes Molekulargewicht: 455 aa, 51 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC068009
• Gene symbol: DDX47
• Gene ID (NCBI): 51202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).