DDX5 Polyklonaler Antikörper

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• KD/KO Validated

DDX5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10804-1-PBS

Synonyme

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  WB analysis of NIH/3T3 using 10804-1-AP (same clone as 10804-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10804-1-AP (same clone as 10804-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5, antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10804-1-AP (same clone as 10804-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10804-1-PBS bindet in WB, IHC, Indirect ELISA DDX5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX5 fusion protein Ag1237
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: BC016027
• Gene symbol: DDX5
• Gene ID (NCBI): 1655
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd