DDX51 Polyklonaler Antikörper

DDX51 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20149-1-AP

Synonyme

EC:3.6.4.13, DEAD box protein 51, ATP-dependent RNA helicase DDX51

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  WB analysis using 20149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20149-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20149-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 20149-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20149-1-AP

  IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

20149-1-AP bindet in WB, IP, ELISA, IF DDX51 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX51 fusion protein Ag13691
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
• Berechnetes Molekulargewicht: 666 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72-80 kDa
• GenBank-Zugangsnummer: BC040185
• Gene symbol: DDX51
• Gene ID (NCBI): 317781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang