DDX51 Polyklonaler Antikörper

DDX51 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20149-1-PBS

Synonyme

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  WB analysis using 20149-1-AP (same clone as 20149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20149-1-AP (same clone as 20149-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 20149-1-AP (same clone as 20149-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 20149-1-AP (same clone as 20149-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 20149-1-AP (same clone as 20149-1-PBS)

  IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20149-1-PBS bindet in WB, IP, Indirect ELISA DDX51 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX51 fusion protein Ag13691
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
• Berechnetes Molekulargewicht: 666 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72-80 kDa
• GenBank-Zugangsnummer: BC040185
• Gene symbol: DDX51
• Gene ID (NCBI): 317781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).