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DDX59 Polyklonaler Antikörper

DDX59 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32948-1-PBS

Synonyme

DEAD box protein 59, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX59, Zinc finger HIT domain-containing protein 5, ZNHIT5

Filter:
  • Western Blot (WB) analysis of various lysates using DDX59 Polyclonal antibody (32948-1-AP)

    WB analysis using 32948-1-AP (same clone as 32948-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32948-1-AP (DDX59 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32948-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX59 Polyclonal antibody (32948-1-AP)

    IHC staining of human breast cancer using 32948-1-AP (same clone as 32948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 32948-1-AP (DDX59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32948-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

32948-1-PBS bindet in WB, IHC, Indirect ELISA DDX59 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DDX59 fusion protein Ag37657
Vollständiger Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
Berechnetes Molekulargewicht 619 aa, 69 kDa
Beobachtetes Molekulargewicht75 kDa
GenBank-ZugangsnummerBC041801
Gene symbol DDX59
Gene ID (NCBI) 83479
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins are multifunctional proteins involved in every aspect in RNA metabolism and have essential roles in many cellular activities. DDX59 is overexpressed in lung adenocarcinoma. DDX59 knockdown reduced cell proliferation, anchorage-independent cell growth, and caused reduction of tumor formation in immunocompromised mice. In multiple lung cancer cells, DDX59 knockdown inhibits DNA synthesis; wild-type DDX59 but not helicase-defective mutant of DDX59 enhances DNA synthesis. DDX59 knockdown caused reduction of MCM protein levels, decreased the loading of MCM ring protein onto chromatin, and therefore inhibited DNA replication. DDX59 has an important role in lung cancer development through promoting DNA replication (PMID: 28090355).