DDX60 Polyklonaler Antikörper

DDX60 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 30982-1-AP

Synonyme

DEAD box protein 60, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX60

---

• 

  WB analysis using 30982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30982-1-AP (DDX60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 30982-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30982-1-AP (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 30982-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30982-1-AP (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human stomach using 30982-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach tissue using DDX60 antibody (30982-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human stomach using 30982-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach tissue using DDX60 antibody (30982-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 30982-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DDX60 antibody (30982-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Magengewebe
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

30982-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA DDX60 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX60 fusion protein Ag34406
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• Berechnetes Molekulargewicht: 1712 aa, 198 kDa
• Beobachtetes Molekulargewicht: 170-200 kDa
• GenBank-Zugangsnummer: BC038115
• Gene symbol: DDX60
• Gene ID (NCBI): 55601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

Publikationen

Species	Application	Title
human	WB	J Inflamm Res; Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis; Authors - Jingjing Xu
human	WB	Front Cell Dev Biol; Genome-wide analysis reveals the MORC3-mediated repression of PD-L1 expression in head and neck cancer; Authors - Wenxuan Fu