DEAF1 Polyklonaler Antikörper

DEAF1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18323-1-AP

Synonyme

DEAF1, NUDR, SPN, Suppressin, ZMYND5

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Galerie der Validierungsdaten

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  WB analysis using 18323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of Jurkat using 18323-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, Jurkat-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Leberkarzinomgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18323-1-AP bindet in WB, IHC, IF, ELISA DEAF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DEAF1 fusion protein Ag13173
• Vollständiger Name: deformed epidermal autoregulatory factor 1 (Drosophila)
• Berechnetes Molekulargewicht: 565 aa, 59 kDa
• Beobachtetes Molekulargewicht: 60-63 kDa, 72 kDa
• GenBank-Zugangsnummer: BC053322
• Gene symbol: DEAF1
• Gene ID (NCBI): 10522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.