DEFA6 Polyklonaler Antikörper

DEFA6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 17923-1-AP

Synonyme

DEF6, Defensin 6, Defensin, alpha 6, Defensin-6, HD 6

---

• 

  WB analysis of COLO 320 using 17923-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17923-1-AP (DEFA6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. (10 Sec exposure).

• 

  WB analysis of mouse small intestine using 17923-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17923-1-AP (DEFA6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

17923-1-AP bindet in WB, IHC, IF, ELISA DEFA6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DEFA6 fusion protein Ag12341
• Vollständiger Name: defensin, alpha 6, Paneth cell-specific
• Berechnetes Molekulargewicht: 100 aa, 11 kDa
• Beobachtetes Molekulargewicht: 6-10 kDa
• GenBank-Zugangsnummer: BC093951
• Gene symbol: DEFA6
• Gene ID (NCBI): 1671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. DEFA6 is alpha 6 of Defensin. This protein is secret expression and can be cleaved in to the fragments with MW 4-6 kDa. It can be used as a maker of Paneth cell. DEFA6 has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. it may protect cells against infection with HIV-1.

Publikationen

Species	Application	Title
Human	IF	J Clin Invest; Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity.; Authors - Kathrin I Liszt
human	IF	Mol Metab; Bitter-tasting drugs tune GDF15 and GLP-1 expression via bitter taste or motilin receptors in the intestine of patients with obesity; Authors - Qian Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Md Zahirul (Verified Customer) (07-23-2025)

For WB, 4hr incubation also showed the same intensity of results as overnight.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for WB, 1:250 for IHC
• Cell Tissue Type: Colorectal Cancer Cells and Patient Derived Organoid