DELE1 Polyklonaler Antikörper

DELE1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21904-1-PBS

Synonyme

DELE, KIAA0141, DAP3-binding cell death enhancer 1, DAP3-binding cell death enhancer 1 short form, DAP3-binding cell death enhancer 1, long form (DELE1(L) )

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  WB analysis of mouse lung using 21904-1-AP (same clone as 21904-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21904-1-AP (KIAA0141 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21904-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 21904-1-AP (same clone as 21904-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21904-1-AP (KIAA0141 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21904-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21904-1-PBS bindet in WB, Indirect ELISA DELE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DELE1 fusion protein Ag14615
• Vollständiger Name: KIAA0141
• Berechnetes Molekulargewicht: 515 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC007855
• Gene symbol: DELE1
• Gene ID (NCBI): 9812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DELE1, also known as KIAA0141, is a 515 amino acid protein, which localizes in Mitochondrion and is detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon. DELE1 is essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation. Mitochondrial malfunction needs to be relayed to the cytosol, where an integrated stress response is triggered by the phosphorylation of eukaryotic translation initiation factor 2α(eIF2α) in mammalian cells. eIF2αphosphorylation is mediated by the four eIF2α kinases GCN2, HRI, PERK and PKR, which are activated by diverse types of cellular stress. OMA1, a mitochondrial stress-activated protease; and DELE1, a little-characterized protein that we found was associated with the inner mitochondrial membrane. Mitochondrial stress stimulates OMA1-dependent cleavage of DELE1 and leads to the accumulation of DELE1 in the cytosol, where it interacts with HRI and activates the eIF2α kinase activity of HRI. In addition, DELE1 is required for ATF4 translation downstream of eIF2α phosphorylation.