DENND1A Polyklonaler Antikörper

DENND1A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25658-1-PBS

Synonyme

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  WB analysis of SKOV-3 using 25658-1-AP (same clone as 25658-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25658-1-AP (same clone as 25658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 25658-1-AP (same clone as 25658-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 25658-1-AP (same clone as 25658-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 25658-1-AP (same clone as 25658-1-PBS)

  IP result of anti-DENND1A (IP:25658-1-AP, 4ug; Detection:25658-1-AP 1:600) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 25658-1-AP (same clone as 25658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 25658-1-AP (same clone as 25658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 25658-1-AP (same clone as 25658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 25658-1-AP (same clone as 25658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SKOV-3 using 25658-1-AP (same clone as 25658-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using DENND1A antibody (25658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25658-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25658-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DENND1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DENND1A fusion protein Ag22547
• Vollständiger Name: DENN/MADD domain containing 1A
• Berechnetes Molekulargewicht: 1009 aa, 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa, 64 kDa
• GenBank-Zugangsnummer: BC039703
• Gene symbol: DENND1A
• Gene ID (NCBI): 57706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.