DENND2D Polyklonaler Antikörper

DENND2D Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33113-1-PBS

Synonyme

DENN Domain Containing 2D , DENN domain-containing protein 2D

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  WB analysis using 33113-1-AP (same clone as 33113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33113-1-AP (DENND2D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 33113-1-AP (same clone as 33113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 33113-1-AP (same clone as 33113-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DENND2D antibody (33113-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

33113-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DENND2D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DENND2D fusion protein Ag36381
• Vollständiger Name: DENN/MADD domain containing 2D
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC004557
• Gene symbol: DENND2D
• Gene ID (NCBI): 79961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DENND2D (DENN domain-containing protein 2D) is a protein containing a DENN domain and belongs to the family of membrane transport proteins, participating in intracellular membrane transport and signal transduction processes. The DENND2D protein comprises several conserved domains, such as the uDENN, DENN, and dDENN domains, which are widely present in various signaling proteins. In cancer research, the expression and methylation status of DENND2D hold significant prognostic value. DENND2D may serve as a novel tumor biomarker for predicting cancer progression and recurrence.