DENR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DENR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 10656-1-AP

Synonyme

SMAP 3, Protein DRP1, H14, DRP1, DRP

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  WB analysis of A431 using 10656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10656-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10656-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10656-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10656-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10656-1-AP

  IP result of anti-DENR (IP:10656-1-AP, 4ug; Detection:10656-1-AP 1:600) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human liver cancer using 10656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10656-1-AP (DENR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10656-1-AP (DENR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10656-1-AP (DENR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10656-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DENR antibody (10656-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10656-1-AP

  Immunofluorescent analysis of A431 cells using 10656-1-AP (DENR antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, Maushirngewebe, Mauslungengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below

Produktinformation

10656-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DENR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DENR fusion protein Ag1023
• Vollständiger Name: density-regulated protein
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC007860
• Gene symbol: DENR
• Gene ID (NCBI): 8562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DENR, also named as SMAP-3 or DRP1, is a 198 amino acid protein, which is highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. DENR is Up-regulated with increasing cell-density by HNRNPD and Up-regulated in ovarian and breast cancer cells by ERBB2 overexpression. The calculated molecular weight of DENR is 22 kDa, but the modified protein is about 25-30 kDa. (PMID: 27239039 )

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human		Cancer Res; MYC Drives mRNA Pseudouridylation to Mitigate Proliferation-Induced Cellular Stress during Cancer Development; Authors - Jane Ding
human	WB	Cell Rep; CRISPR screening reveals a dependency on ribosome recycling for efficient SARS-CoV-2 programmed ribosomal frameshifting and viral replication; Authors - Frederick Rehfeld ; KD Validated
human	WB	Sci Rep; A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties.; Authors - Mohamad A Zaini ; KO Validated
zebrafish	WB	Nutrients; A High-Fat Diet Induces Muscle Mitochondrial Dysfunction and Impairs Swimming Capacity in Zebrafish: A New Model of Sarcopenic Obesity.; Authors - Yun-Yi Zou
rat	WB	J Cell Mol Med; A possible new activator of PI3K-Huayu Qutan Recipe alleviates mitochondrial apoptosis in obesity rats with acute myocardial infarction.; Authors - He Tai