DEPDC6 Polyklonaler Antikörper

DEPDC6 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21312-1-PBS

Synonyme

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  WB analysis of HeLa using 21312-1-AP (same clone as 21312-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 21312-1-AP (same clone as 21312-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 21312-1-AP (same clone as 21312-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21312-1-PBS bindet in WB, IHC, Indirect ELISA DEPDC6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DEPDC6 fusion protein Ag15959
• Vollständiger Name: DEP domain containing 6
• Berechnetes Molekulargewicht: 409 aa, 46 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC024746
• Gene symbol: DEPDC6
• Gene ID (NCBI): 64798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.