DFFA/DFF45 Monoklonaler Antikörper

DFFA/DFF45 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6H9

Kat-Nr. : 68177-1-PBS

Synonyme

DFFA,DFF45, DFF45, DFFA, 1F6H9, DFF alpha

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  WB analysis using 68177-1-Ig (same clone as 68177-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68177-1-Ig (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DFFA,DFF45 antibody (68177-1-Ig, Clone: 1F6H9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68177-1-Ig (same clone as 68177-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug DFFA,DFF45 Monoclonal antibody (68177-1-Ig, Clone:1F6H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68177-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50354-1

  Cytometric bead array standard curve of MP50354-1, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-1-PBS. Detection antibody: 68177-2-PBS. Standard:Ag32657. Range: 3.125-200 ng/mL.

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  Cytometric bead array standard curve of MP50354-2

  Cytometric bead array standard curve of MP50354-2, DFFA,DFF45 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68177-3-PBS. Detection antibody: 68177-1-PBS. Standard:Ag32657. Range: 6.25-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68177-1-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA DFFA/DFF45 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DFFA/DFF45 fusion protein Ag32657
• Vollständiger Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC000037
• Gene symbol: DFFA
• Gene ID (NCBI): 1676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.