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DFFA,DFF45 Rekombinanter Antikörper

DFFA,DFF45 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242915D6

Kat-Nr. : 85220-5-PBS

Synonyme

DFF45, DFFA, 242915D6, DFF alpha, DFF1

Filter:
  • Western Blot (WB) analysis of various lysates using DFFA,DFF45 Recombinant antibody (85220-5-RR)

    WB analysis using 85220-5-RR (same clone as 85220-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DFFA,DFF45 Recombinant antibody (85220-5-RR)

    IF Staining of HeLa using 85220-5-RR (same clone as 85220-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85220-5-RR

    Affinity and Kinetic Characterization of 85220-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

Geprüfte Anwendungen

Produktinformation

85220-5-PBS bindet in WB, IF/ICC, Indirect ELISA DFFA,DFF45 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DFFA,DFF45 fusion protein Ag0244
Vollständiger Name DNA fragmentation factor, 45kDa, alpha polypeptide
Berechnetes Molekulargewicht 37 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC000037
Gene symbol DFFA
Gene ID (NCBI) 1676
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.