DFFA,DFF45 Rekombinanter Antikörper

DFFA,DFF45 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242915D6

Kat-Nr. : 85220-5-RR

Synonyme

DFF45, DFFA, 242915D6, DFF alpha, DFF1

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  WB analysis using 85220-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85220-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85220-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, C6-Zellen, HEK-293-Zellen, K-562-Zellen, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85220-5-RR bindet in WB, IF/ICC, ELISA DFFA,DFF45 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DFFA,DFF45 fusion protein Ag0244
• Vollständiger Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000037
• Gene symbol: DFFA
• Gene ID (NCBI): 1676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.