DFNA5/GSDME Polyklonaler Antikörper

DFNA5/GSDME Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31363-1-PBS

Synonyme

DFNA5, Gasdermin E, Gasdermin-E, GSDME, deafness, autosomal dominant 5

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  WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

  Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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  WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 31363-1-AP (same clone as 31363-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

  IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DFNA5 antibody (31363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31363-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DFNA5/GSDME und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DFNA5/GSDME fusion protein Ag35186
• Vollständiger Name: deafness, autosomal dominant 5
• Berechnetes Molekulargewicht: 496 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 35 kDa, 25 kDa
• GenBank-Zugangsnummer: BC019689
• Gene symbol: DFNA5
• Gene ID (NCBI): 1687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.