DFNA5/GSDME Rekombinanter Antikörper

DFNA5/GSDME Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240394D2

Kat-Nr. : 83454-6-RR

Synonyme

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 240394D2

---

• 

  WB analysis using 83454-6-RR

  Untreated and etoposide-treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83454-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 83454-6-RR

  IP result of anti-DFNA5/GSDME (IP:83454-6-RR, 4ug; Detection:83454-6-RR 1:1000) with SH-SY5Y cells lysate 1840 ug.

• 

  Affinity and Kinetic Characterization of 83454-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83454-6-RR against Human DFNA5 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83454-6-RR bindet in WB, IP, ELISA DFNA5/GSDME und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DFNA5/GSDME fusion protein Ag3746
• Vollständiger Name: deafness, autosomal dominant 5
• Berechnetes Molekulargewicht: 496 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 35 kDa
• GenBank-Zugangsnummer: BC019689
• Gene symbol: DFNA5
• Gene ID (NCBI): 1687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.