DFNA5/GSDME Monoklonaler Antikörper

DFNA5/GSDME Monoklonal Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D9C3

Kat-Nr. : 67731-1-PBS

Synonyme

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3

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  WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67731-1-Ig (same clone as 67731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67731-1-Ig (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 67731-1-Ig (same clone as 67731-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human DFNA5/GSDME (67731-1-Ig, Clone:1D9C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67731-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67731-1-PBS bindet in WB, FC (Intra), Indirect ELISA DFNA5/GSDME und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DFNA5/GSDME fusion protein Ag30514
• Vollständiger Name: deafness, autosomal dominant 5
• Berechnetes Molekulargewicht: 496 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC019689
• Gene symbol: DFNA5
• Gene ID (NCBI): 1687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.