DGAT1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

DGAT1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

230091E1

Kat-Nr. : 82945-1-RR

Synonyme

ACAT related gene product 1, AGRP1, ARGP1, DGAT, DGAT1, Diglyceride acyltransferase

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  WB analysis using 82945-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82945-1-RR (DGAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 82945-1-RR

  WB result of DGAT1 antibody (82945-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DGAT1 transfected HeLa cells.

• 

  WB analysis using 82945-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82945-1-RR (DGAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen, HepG2-Zellen, Mauslebergewebe, PC-3-Zellen, Rattenlebergewebe, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

82945-1-RR bindet in WB, ELISA DGAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DGAT1 fusion protein Ag32717
• Vollständiger Name: diacylglycerol O-acyltransferase homolog 1 (mouse)
• Berechnetes Molekulargewicht: 488 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC015762
• Gene symbol: DGAT1
• Gene ID (NCBI): 8694
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Phytomedicine; Integrated metabolomics and mass spectrometry imaging analysis reveal the efficacy and mechanism of Huangkui capsule on type 2 diabetic nephropathy; Authors - Jinwei Han
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Inducible global knockout of surfeit locus protein 4 in adult mice results in hypolipidemia, intestinal lipid accumulation, liver injury, and increased mortality; Authors - Wei Chen
human	WB	FEBS J; Human transmembrane protein 68 links triacylglycerol synthesis to membrane lipid homeostasis; Authors - Fansi Zeng