DGCR8 C-terminal Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DGCR8 C-terminal Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

84

Kat-Nr. : 10996-1-AP

Synonyme

C22orf12, DGCR8, DGCR8 C-terminal, DGCRK6, Gy1

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Galerie der Validierungsdaten

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  WB analysis using 10996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10996-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10996-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10996-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10996-1-AP

  IP Result of anti-DGCR8 C-terminal (IP:10996-1-AP, 3ug; Detection:10996-1-AP 1:800) with HEK-293 cells lysate 2700ug.

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 10996-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 10996-1-AP

  1X10^6 HeLa cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

• 

  FC experiment of SH-SY5Y using 10996-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 74 publications below
• IHC: See 5 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below
• RIP: See 4 publications below

Produktinformation

10996-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA DGCR8 C-terminal und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGCR8 C-terminal fusion protein Ag1429
• Vollständiger Name: DiGeorge syndrome critical region gene 8
• Berechnetes Molekulargewicht: 773 aa, 86 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC009323
• Gene symbol: DGCR8
• Gene ID (NCBI): 54487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

Publikationen

Species	Application	Title
mouse,human	WB,IF	Nature; Global miRNA dosage control of embryonic germ layer specification.; Authors - Yingzi Cui ; KO Validated
human	WB	Nature; Modulation of microRNA processing by p53.; Authors - Suzuki Hiroshi I HI
human	WB	Cell; Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.; Authors - Piskounova Elena E
human	WB	Cell; Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer.; Authors - Masaki Mori
mouse	ChIP	Cell; Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis.; Authors - Hiroshi I Suzuki
human	WB	Cell Stem Cell; Principles of signaling pathway modulation for enhancing human naive pluripotency induction.; Authors - Jonathan Bayerl

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