DGCR8 N-terminal Polyklonaler Antikörper

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• KD/KO Validated

DGCR8 N-terminal Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 25835-1-AP

Synonyme

C22orf12, DGCR8, DGCRK6, Gy1

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Galerie der Validierungsdaten

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  WB analysis of A431 cells using 25835-1-AP

  WB result of DGCR8 antibody (25835-1-AP, 1:2000) with si-control and si-DGCR8 transfected A431 cells.

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  WB analysis of HeLa using 25835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25835-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25835-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25835-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25835-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 25835-1-AP

  IP Result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.

• 

  IF Staining of HeLa using 25835-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

25835-1-AP bindet in WB, IP, IF, ELISA DGCR8 N-terminal und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGCR8 N-terminal fusion protein Ag22988
• Vollständiger Name: DiGeorge syndrome critical region gene 8
• Berechnetes Molekulargewicht: 773 aa, 86 kDa
• Beobachtetes Molekulargewicht: 88-100 kDa
• GenBank-Zugangsnummer: BC078147
• Gene symbol: DGCR8
• Gene ID (NCBI): 54487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Publikationen

Species	Application	Title
mouse	IF	Nucleic Acids Res; DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity; Authors - An Yan
bovine	IF	Proc Natl Acad Sci U S A; Embryonic microRNAs are essential for bovine preimplantation embryo development; Authors - Erika E Paulson