DGCR8 Monoklonaler Antikörper

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• KD/KO Validated

DGCR8 Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, RIP, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

CloneNo.

4D11A3

Kat-Nr. : 60084-1-Ig

Synonyme

C22orf12, DGCR8, DGCRK6, Gy1

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Galerie der Validierungsdaten

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  WB analysis using 60084-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60084-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60084-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60084-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 60084-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 60084-1-Ig

  IP Result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, BxPC-3-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

60084-1-Ig bindet in WB, RIP, IP, ELISA DGCR8 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DGCR8 fusion protein Ag4871
• Vollständiger Name: DiGeorge syndrome critical region gene 8
• Berechnetes Molekulargewicht: 773 aa, 86 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC009323
• Gene symbol: DGCR8
• Gene ID (NCBI): 54487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Publikationen

Species	Application	Title
mouse,human	WB	Nature; Global miRNA dosage control of embryonic germ layer specification.; Authors - Yingzi Cui ; KO Validated
human	WB	Nat Commun; Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.; Authors - Hailong Zhang
human,rat	WB	Aging Cell; p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease; Authors - Haidong Xu
human	WB,RIP	Mol Ther; DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.; Authors - Fangfang Zhang
human, mouse	WB	Nucleic Acids Res; SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.; Authors - Changhong Zhu
human	WB	Oncogene; Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.; Authors - Peric D D