DGCR8 Monoklonaler Antikörper

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DGCR8 Monoklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4D11A3

Kat-Nr. : 60084-1-PBS

Synonyme

C22orf12, DGCR8, DGCRK6, Gy1

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  WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60084-1-Ig (same clone as 60084-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60084-1-Ig (same clone as 60084-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60084-1-Ig (same clone as 60084-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 60084-1-Ig (same clone as 60084-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

  IP result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60084-1-PBS bindet in WB, IP, Indirect ELISA DGCR8 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DGCR8 fusion protein Ag4871
• Vollständiger Name: DiGeorge syndrome critical region gene 8
• Berechnetes Molekulargewicht: 773 aa, 86 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC009323
• Gene symbol: DGCR8
• Gene ID (NCBI): 54487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.