DGKA Polyklonaler Antikörper

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• KD/KO Validated

DGKA Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 11547-1-AP

Synonyme

80 kDa diacylglycerol kinase, DAG kinase alpha, DAGK, DAGK1, DGK alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha

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Galerie der Validierungsdaten

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  WB analysis using 11547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 11547-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11547-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 11547-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11547-1-AP

  IP Result of anti-DGKA (IP:11547-1-AP, 3ug; Detection:11547-1-AP 1:1000) with Jurkat cells lysate 1500ug.

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  IHC staining of human gliomas using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11547-1-AP (DGKA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11547-1-AP (DGKA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 11547-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of Jurkat using 11547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DGKA antibody (11547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Jurkat using 11547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DGKA antibody (11547-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 11547-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DGKA (11547-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Cerebellum-Gewebe, Maus-Cerebellum-Gewebe, MOLT-4-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Jurkat-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11547-1-AP bindet in WB, IP, IHC, IF, FC, ELISA DGKA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGKA fusion protein Ag2075
• Vollständiger Name: diacylglycerol kinase, alpha 80kDa
• Berechnetes Molekulargewicht: 735 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC023523
• Gene symbol: DGKA
• Gene ID (NCBI): 1606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

Publikationen

Species	Application	Title
		Nat Commun; Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.; Authors - David Novo
human	WB	Nat Chem Biol; Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.; Authors - Timothy B Ware ; KD Validated
human	WB	Nat Commun; Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.; Authors - Christoph Weigel
human	WB	Cancer Discov; Diacylglycerol Kinase α Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers.; Authors - Dominguez Charli L CL ; KD Validated
human	WB,IHC	Clin Cancer Res; DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.; Authors - Jie Li ; KD Validated
mouse	WB	J Immunother Cancer; Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes.; Authors - Javier Arranz-Nicolás