DGKE Polyklonaler Antikörper

DGKE Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11900-1-PBS

Synonyme

---

• 

  WB analysis using 11900-1-AP (same clone as 11900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11900-1-AP (DGKE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 11900-1-AP (same clone as 11900-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKE antibody (11900-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11900-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DGKE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGKE fusion protein Ag2512
• Vollständiger Name: diacylglycerol kinase, epsilon 64kDa
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 60-64 kDa
• GenBank-Zugangsnummer: BC022297
• Gene symbol: DGKE
• Gene ID (NCBI): 8526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGKE(Diacylglycerol kinase epsilon) is also named as DAGK5 and belongs to the eukaryotic diacylglycerol kinase family.It may be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction(PMID:16689942).When expressed in mammalian cells, DGK-epsilon showed specificity for arachidonyl-containing diacylglycerol.