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DGKK-Specific Polyklonaler Antikörper

DGKK-Specific Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20090-1-PBS

Synonyme

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  • Western Blot (WB) analysis of human testis tissue using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of human testis using 20090-1-AP (same clone as 20090-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of human testis using 20090-1-AP (same clone as 20090-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of HuH-7 using 20090-1-AP (same clone as 20090-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DGKK-Specific Polyclonal antibody (20090-1-AP)

    IF Staining of HepG2 using 20090-1-AP (same clone as 20090-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20090-1-PBS bindet in WB, IF/ICC, Indirect ELISA DGKK-Specific und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name diacylglycerol kinase, kappa
Berechnetes Molekulargewicht 142 kDa
Beobachtetes Molekulargewicht 135-140 kDa
GenBank-ZugangsnummerNM_001013742
Gene symbol DGKK
Gene ID (NCBI) 139189
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.