DGKZ Rekombinanter Antikörper

DGKZ Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241444A2

Kat-Nr. : 84830-5-PBS

Synonyme

241444A2, DAG kinase zeta, DAGK5, DAGK6, DGK ZETA

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  WB analysis using 84830-5-RR (same clone as 84830-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84830-5-RR (DGKZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84830-5-RR (same clone as 84830-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84830-5-RR (same clone as 84830-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84830-5-RR (same clone as 84830-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKZ antibody (84830-5-RR, Clone: 241444A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84830-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DGKZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DGKZ fusion protein Ag37336
• Vollständiger Name: diacylglycerol kinase, zeta 104kDa
• Berechnetes Molekulargewicht: 104kDa，928aa
• Beobachtetes Molekulargewicht: 104-124 kDa
• GenBank-Zugangsnummer: NM_001199267.1
• Gene symbol: DGKZ
• Gene ID (NCBI): 8525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Diacylglycerol kinase zeta (DGKZ), also known as DAGK5, DAGK6, DGK-ZETA, hDGKzeta, belongs to the diacylglycerol kinase (DGK) family which catalyzes diacylglycerol (DAG) into phosphatidic acid (PA). DGKZ is a novel promoter of metastasis and that it could be a potential prognostic indicator in patients with triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFbetaR2(PMID: 35115500). Downregulation of DGKZ suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest(PMID: 33926342).