dGK Polyklonaler Antikörper

dGK Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16314-1-AP

Synonyme

DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial

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  WB analysis of mouse kidney using 16314-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16314-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 16314-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 16314-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 16314-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16314-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16314-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16314-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16314-1-AP bindet in WB, IHC, ELISA dGK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: dGK fusion protein Ag9361
• Vollständiger Name: deoxyguanosine kinase
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa, 56-64 kDa
• GenBank-Zugangsnummer: BC015757
• Gene symbol: DGUOK
• Gene ID (NCBI): 1716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Deoxyguanosine kinase (dGK) deficiency is a frequent cause of mitochondrial DNA depletion associated with a hepatocerebral phenotype. dGK is a member of the deoxyribonucleoside kinase family involved in phosphorylation of deoxyribonucleosides. In mammals, there are four deoxyribonucleoside kinases with overlapping specificities: two cytoplasmic enzymes, thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK), and two mitochondrial enzymes, thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK). The human dGK protein is highly specific for purine substrates. Human dGK is a dimer of two identical subunits of 30 kDa. The three-dimensional structure of the human dGK monomer has been reported, providing an explanation for the substrate specificity of the enzyme. (PMID: 29786478, PMID: 11427893, PMID: 17073823)