DHCR24 Polyklonaler Antikörper

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• KD/KO Validated

DHCR24 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 10471-1-AP

Synonyme

DHC24, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol Delta-24-reductase, Delta(24)-sterol reductase, EC:1.3.1.72

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  WB analysis using 10471-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10471-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10471-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10471-1-AP (DHCR24 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, L02-Zellen, MCF-7-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Mammakarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below

Produktinformation

10471-1-AP bindet in WB, IHC, ELISA DHCR24 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHCR24 fusion protein Ag0756
• Vollständiger Name: 24-dehydrocholesterol reductase
• Berechnetes Molekulargewicht: 60 kDa, 516 aa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC004375
• Gene symbol: DHCR24
• Gene ID (NCBI): 1718
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Publikationen

Species	Application	Title
mouse,human	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	EMBO Mol Med; Shaping immune landscape of colorectal cancer by cholesterol metabolites; Authors - Yibing Bai
human	WB	Cell Death Differ; Efferocytosis potentiates the expression of arachidonate 15-lipoxygenase (ALOX15) in alternatively activated human macrophages through LXR activation.; Authors - Ryan G Snodgrass
human	WB	Commun Biol; Cholesterol mediated ferroptosis suppression reveals essential roles of Coenzyme Q and squalene; Authors - Qi Sun ; KD Validated
		J Neurosci; Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.; Authors - Cortnie Hartwig
mouse	WB	NanoImpact; The alterations of miRNA and mRNA expression profile and their integration analysis induced by silica nanoparticles in spermatocyte cells.; Authors - Guiqing Zhou

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