DHCR24 Monoklonaler Antikörper

DHCR24 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6E11

Kat-Nr. : 60028-1-PBS

Synonyme

DHC24, 24-dehydrocholesterol reductase, 2F6E11, 3-beta-hydroxysterol Delta-24-reductase, Delta(24)-sterol reductase

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  WB analysis of HepG2 using 60028-1-Ig (same clone as 60028-1-PBS)

  WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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  WB analysis using 60028-1-Ig (same clone as 60028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60028-1-Ig (same clone as 60028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60028-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51020-1

  Cytometric bead array standard curve of MP51020-1, DHCR24 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60028-2-PBS. Detection antibody: 60028-1-PBS. Standard:Ag0756. Range: 0.098-100 ng/mL.

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  Affinity and Kinetic Characterization of 60028-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60028-1-Ig against Human DHCR24 were performed. The affinity constant is 23 nM.

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Geprüfte Anwendungen

Produktinformation

60028-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA DHCR24 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DHCR24 fusion protein Ag0756
• Vollständiger Name: 24-dehydrocholesterol reductase
• Berechnetes Molekulargewicht: 60 kDa, 516 aa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC004375
• Gene symbol: DHCR24
• Gene ID (NCBI): 1718
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).